Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
Regular Papers
Analysis of Ammonia Monooxygenase and Archaeal 16S rRNA Gene Fragments in Nitrifying Acid-Sulfate Soil Microcosms
Asami NakayaYuki OnoderaTatsunori NakagawaKazuo SatohReiji TakahashiSatohiko SasakiTatsuaki Tokuyama
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Volume 24 (2009) Issue 2 Pages 168-174

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The present study describes the occurrence of a unique archaeal ammonia monooxygenase alpha subunit (amoA) gene in nitrifying acid-sulfate soil microcosms at pH 3.5. The soil was collected from an abandoned paddy field in Thailand. Microcosms were incubated in the dark at 30°C for 372 days with the following three treatments: addition of ammonium sulfate solution once a month (I) or once a week (II), and addition of only sterilized water (III). A quantitative PCR analysis revealed an increase in abundance of the archaeal amoA gene in microcosm soils in which nitrate concentrations increased after incubation. A phylogenetic analysis indicated a predominance of the novel gene, and a predominance of a betaproteobacterial amoA gene affiliated with the genus Nitrosospira. A 16S rRNA gene-based PCR assay revealed that crenarchaeotic Group I.1d was predominant among the Crenarchaeota in microcosms. These results suggest the presence of ammonia-oxidizing archaea corresponding to the unique amoA lineage in nitrifying acid-sulfate soil microcosms at pH 3.5.

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© Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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