Specific and Rapid Quantification of Viable Listeria monocytogenes Using Fluorescence in situ Hybridization in Combination with Filter Cultivation

Ikufumi Fuchizawa; Shigemasa Shimizu; Masashi Ootsubo; Yuji Kawai; Koji Yamazaki

doi:10.1264/jsme2.ME09102

Short Communications

Specific and Rapid Quantification of Viable Listeria monocytogenes Using Fluorescence in situ Hybridization in Combination with Filter Cultivation

Ikufumi Fuchizawa, Shigemasa Shimizu, Masashi Ootsubo, Yuji Kawai, Koji Yamazaki

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Keywords: Listeria monocytogenes, fluorescence in situ hybridization, specific detection, FISHFC

JOURNAL FREE ACCESS

2009 Volume 24 Issue 3 Pages 273-275

DOI https://doi.org/10.1264/jsme2.ME09102

View "Advance Publication" version (May 28, 2009).

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Abstract

We developed a new method that rapidly and specifically enumerates only viable Listeria monocytogenes in food using fluorescence in situ hybridization in combination with filter cultivation (FISHFC). Viable L. monocytogenes could be specifically quantified within 16 h using an Alexa647-labeled mRL-2 probe. The coefficient of the correlation between the new method and the conventional plating method was 0.959.

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