Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Short Communications
Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining
Takashi BabaNaoko InoueNobuyasu YamaguchiMasao Nasu
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2012 Volume 27 Issue 3 Pages 324-326


In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water samples collected from 30 bathing facilities, with similar sensitivity of a conventional plate-counting method. These results suggest that the microcolony method combined with fluorescent antibody staining could be useful as a monitoring technique for the prevention of Legionnaires’ disease through the early detection of L. pneumophila in freshwater.

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© 2012 Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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