Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Short Communications
Eukaryotic Microbiomes of Membrane-Attached Biofilms in Membrane Bioreactors Analyzed by High-Throughput Sequencing and Microscopic Observations
Tomohiro InabaTomoyuki HoriYuya SatoTomo AoyagiDai HanajimaAtsushi OgataHiroshi Habe
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Supplementary material

2018 Volume 33 Issue 1 Pages 98-101

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Abstract

Limited information is currently available on the contribution of eukaryotes to the reactor performance of membrane bioreactors (MBRs). Using high-throughput Illumina sequencing of 18S rRNA genes and microscopic observations, we investigated eukaryotic microbiomes in membrane-attached biofilms in MBRs treating piggery wastewater. Protozoa preying on bacteria were frequently detected under stable conditions when membrane clogging was suppressed. However, the eukaryotes preying upon protozoa became predominant in biofilms when membrane fouling rapidly progressed. We herein demonstrated that a comprehensive investigation of eukaryotic microbiomes using high-throughput sequencing contributes to a better understanding of the microbial ecology involved in wastewater treatment.

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© 2018 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions.
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