Visualizing the Effects of Biofilm Structures on the Influx of Fluorescent Material Using Combined Confocal Reflection and Fluorescent Microscopy

Abstract

Mass transport into the interior of biofilms and biological aggregates is a critical factor that affects their metabolic activity. In this study, we demonstrated the utility of a simple procedure that combines confocal reflection microscopy and fluorescent confocal laser scanning microscopy to visually explain the effects of biofilm structure on mass transport.