Detection of vanA-containing Enterococcus Species in Faecal Microbiota of Gilthead Seabream (Sparus aurata)

Joana Barros; Margarida Andrade; Hajer Radhouani; Maria López; Gilberto Igrejas; Patricia Poeta; Carmen Torres

doi:10.1264/jsme2.ME11346

This article has now been updated. Please use the final version.

Detection of vanA-containing Enterococcus Species in Faecal Microbiota of Gilthead Seabream (Sparus aurata)

Joana Barros, Margarida Andrade, Hajer Radhouani ^†, Maria López ^†, Gilberto Igrejas, Patricia Poeta, Carmen Torres

Author information

Joana Barros
Center of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro
Veterinary Science Department, University of Trás-os-Montes and Alto Douro
Margarida Andrade
Center of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro
Veterinary Science Department, University of Trás-os-Montes and Alto Douro
Hajer Radhouani ^†
Center of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro
Veterinary Science Department, University of Trás-os-Montes and Alto Douro
Institut for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro
Center of Studies of Animal and Veterinary Sciences
Maria López ^†
Biochemistry and Molecular Biology Area, University of La Rioja
Gilberto Igrejas
Center of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro
Institut for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro
Patricia Poeta
Veterinary Science Department, University of Trás-os-Montes and Alto Douro
Center of Studies of Animal and Veterinary Sciences
Carmen Torres
Biochemistry and Molecular Biology Area, University of La Rioja
Author contributions

†These authors contributed equally: Hajer Radhouani, Maria López

Keywords: Enterococcus, vanA, MLST, fish

JOURNAL FREE ACCESS Advance online publication

Article ID: ME11346

DOI https://doi.org/10.1264/jsme2.ME11346

The final version of this article is now available: Vol. 27 (2009), No. 4

Details

Abstract

Vancomycin-resistant Enterococcus faecalis, E. faecium and E. durans isolates with the genotype vanA were detected in 7 of 118 faecal samples (5.9%) of natural gilthead seabream recovered off the coast of Portugal, and one vancomycin-resistant isolate/sample was further characterized. The genes erm(B), tet(L), tet(M), aac(6´)-aph(2"), aph(3´)-IIIa and/or ant(6)-Ia were identified in most of the 7 vancomycin-resistant enterococci. Sequence types ST273, ST313 and ST76 were detected in three E. faecium isolates and ST6 in two E. faecalis isolates. VanA-containing enterococci are suggested to be disseminated in fish in marine ecosystems close to areas of human activity.

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