In Situ Gene Expression Responsible for Sulfide Oxidation and CO2 Fixation of an Uncultured Large Sausage-Shaped Aquificae Bacterium in a Sulfidic Hot Spring

Satoshi Tamazawa; Kyosuke Yamamoto; Kazuto Takasaki; Yasuo Mitani; Satoshi Hanada; Yoichi Kamagata; Hideyuki Tamaki

doi:10.1264/jsme2.ME16013

This article has now been updated. Please use the final version.

In Situ Gene Expression Responsible for Sulfide Oxidation and CO₂ Fixation of an Uncultured Large Sausage-Shaped Aquificae Bacterium in a Sulfidic Hot Spring

Satoshi Tamazawa, Kyosuke Yamamoto, Kazuto Takasaki, Yasuo Mitani, Satoshi Hanada, Yoichi Kamagata, Hideyuki Tamaki

Author information

Satoshi Tamazawa
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Kyosuke Yamamoto
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Kazuto Takasaki
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Yasuo Mitani
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Satoshi Hanada
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Graduate School of Life and Environmental Sciences, University of Tsukuba
Yoichi Kamagata
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Hideyuki Tamaki
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Keywords: sulfur-turf microbial mats, uncultured Aquificae bacterium, in situ gene expression, sulfide oxidation, primary production

JOURNAL FREE ACCESS Advance online publication

Article ID: ME16013

DOI https://doi.org/10.1264/jsme2.ME16013

The final version of this article is now available: Vol. 31 (2016), No. 2 pp. 194-198

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Abstract

We investigated the in situ gene expression profile of sulfur-turf microbial mats dominated by an uncultured large sausage-shaped Aquificae bacterium, a key metabolic player in sulfur-turfs in sulfidic hot springs. A reverse transcription-PCR analysis revealed that the genes responsible for sulfide, sulfite, and thiosulfate oxidation and carbon fixation via the reductive TCA cycle were continuously expressed in sulfur-turf mats taken at different sampling points, seasons, and years. These results suggest that the uncultured large sausage-shaped bacterium has the ability to grow chemolithoautotrophically and plays key roles as a primary producer in the sulfidic hot spring ecosystem in situ.

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