Presence of Cu-Type (NirK) and cd1-Type (NirS) Nitrite Reductase Genes in the Denitrifying Bacterium Bradyrhizobium nitroreducens sp. nov.

Jeonghwan Jang; Naoaki Ashida; Ayaaki Kai; Kazuo Isobe; Tomoyasu Nishizawa; Shigeto Otsuka; Akira Yokota; Keishi Senoo; Satoshi Ishii

doi:10.1264/jsme2.ME18039

Presence of Cu-Type (NirK) and cd₁-Type (NirS) Nitrite Reductase Genes in the Denitrifying Bacterium Bradyrhizobium nitroreducens sp. nov.

Jeonghwan Jang, Naoaki Ashida, Ayaaki Kai, Kazuo Isobe, Tomoyasu Nishizawa, Shigeto Otsuka, Akira Yokota, Keishi Senoo, Satoshi Ishii

Author information

Jeonghwan Jang
Biotechnology Institute, University of Minnesota
Naoaki Ashida
Department of Applied Biological Chemistry, The University of Tokyo
Ayaaki Kai
Department of Applied Biological Chemistry, The University of Tokyo
Kazuo Isobe
Department of Applied Biological Chemistry, The University of Tokyo
Tomoyasu Nishizawa
Department of Food and Life Sciences, Ibaraki University College of Agriculture
Shigeto Otsuka
Department of Applied Biological Chemistry, The University of Tokyo
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo
Akira Yokota
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo
Division of Bioscience and Biotechnology for Future Bioindustries, Graduate School of Agricultural Sciences, Tohoku University
Keishi Senoo
Department of Applied Biological Chemistry, The University of Tokyo
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo
Satoshi Ishii
Biotechnology Institute, University of Minnesota
Department of Soil, Water, and Climate, University of Minnesota

Keywords: denitrification, genome, Bradyrhizobium, new species

JOURNALS FREE ACCESS Advance online publication

Article ID: ME18039

DOI https://doi.org/10.1264/jsme2.ME18039

The final version of this article with its full bibliographical information is available. To access the article, click here (Vol. 33 (2018), No. 3 pp. 326-331).

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Abstract

Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd₁-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1^T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1^T (=JCM 18858^T=KCTC 62391^T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

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