Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis

Shun Kobayashi; Daisuke Hira; Keitaro Yoshida; Masanori Toyofuku; Yosuke Shida; Wataru Ogasawara; Takashi Yamaguchi; Nobuo Araki; Mamoru Oshiki

doi:10.1264/jsme2.ME18058

This article has now been updated. Please use the final version.

Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis

Shun Kobayashi, Daisuke Hira, Keitaro Yoshida, Masanori Toyofuku, Yosuke Shida, Wataru Ogasawara, Takashi Yamaguchi, Nobuo Araki, Mamoru Oshiki

Author information

Shun Kobayashi
Department of Civil Engineering, National Institute of Technology, Nagaoka College
Daisuke Hira
Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University
Keitaro Yoshida
Graduate School of Life and Environmental Sciences, University of Tsukuba
Masanori Toyofuku
Graduate School of Life and Environmental Sciences, University of Tsukuba
Yosuke Shida
Department of Bioengineering, Nagaoka University of Technology
Wataru Ogasawara
Department of Bioengineering, Nagaoka University of Technology
Takashi Yamaguchi
Department of Science of Technology Innovation, Nagaoka University of Technology
Nobuo Araki
Department of Civil Engineering, National Institute of Technology, Nagaoka College
Mamoru Oshiki
Department of Civil Engineering, National Institute of Technology, Nagaoka College

Keywords: nitrite reduction, hydroxylamine oxidation, nitrous oxide production, ammonia oxidizing archaea, Nitrososphaera viennensis

JOURNAL FREE ACCESS Advance online publication

Article ID: ME18058

DOI https://doi.org/10.1264/jsme2.ME18058

The final version of this article is now available: Vol. 33 (2018), No. 4 pp. 428-434

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Abstract

Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite reductase (NirK) from Nitrososphaera viennensis and investigated its enzymatic properties. The recombinant protein catalyzed the reduction of ¹⁵NO₂^– to ¹⁵NO, the oxidation of hydroxylamine (¹⁵NH₂OH) to ¹⁵NO, and the production of ^14-15N₂O from ¹⁵NH₂OH and ¹⁴NO₂^–. To the best of our knowledge, the present study is the first to document the enzymatic properties of AOA NirK.

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