Abstract
Mechanical vibration at low amplitude and high frequency is a promising method to prevent osteoporotic bone loss. However, optimal conditions of the vibration have not been fully elucidated. Therefore, the aim of this study was to clarify the optimal amplitude and frequency that enhances bone metabolism. Osteocytic cell line MLO-Y4 cells were subjected to vertical vibration at magnitude of 20-100 μm and frequency of 10-100 Hz. Secretion of nitric oxide (NO) from the cells was evaluated using fluorescent indicator, DAF-2DA. Quantitative comparison of fluorescent intensity showed that NO secretion was significantly increased when the cells were exposed to the vibration at the magnitude of 40-100 μm and the frequency of 20-100 Hz. The most effective conditions of mechanical vibration were 60 μm in amplitude and 20 Hz in frequency.
