Abstract
Head impact may causes hemorrhage and brain edema. These are thought to be related to impulsive pressure in the skull, but that hasn't been investigated in detail. Therefore, the purpose of this study was to clarify relationship between impulsive pressure and hemorrhage and brain edema. Brain edema is considered to be due to disruption of blood brain barrier (BBB). BBB is a selective permeability barrier. BBB is formed by endothelial cells, pericytes and astrocytes. In this report, we describe about relationship between impulsive pressure and disruption of BBB. Endothelial cells are connected by adhesion proteins. When adhesion protein decrease, blood plasma leak out from BBB and cause brain edema. Impulsive pressure is reproduced using the device loading impulsive pressure. BBB is reproduced using in vitro BBB models. I used two types of models (E00: Endothelial cells, EPA: Endothelial cells, pericytes and astrocytes). I expose impulsive pressure to in vitro BBB models. After that, we measured the transendothelial electrical resistance (TEER) for estimate effect of impulsive pressure. TEER depends on the expression of cell adhesion protein. In the result, TEER decreased after loading impulsive pressure. Decrease of TEER in EPA model is larger than E00 model. These results suggest that impulsive pressure decrease the expression of adhesion protein. The expression of adhesion protein causes brain edema. These results also suggest that pericytes and astrocytes have an effect on permeability of BBB after loading impulsive pressure.
