1H25 Uterus Tissue Engineering by the matrices decellularized with hyper hydrostatic pressure

Abstract

To recover serious uterine damages which cause infertility, tissue engineering is a promising method. However, there is a lack of effective models of the regenerative tissues which can be utilized in clinical application. In such a situation, we have noted the effectiveness of decellularized tissues, which are produced from native tissues by removing cells which cause an immune reaction. Moreover, it is assumed the decellularized tissues are useful also in their mechanical characteristic because they remain the structure of native tissues. We have reported that the tissue decellularized with hyper hydrostatic pressure and then implanted in a defective part of native rat uterus encourages the regeneration of the tissues, and this study is performed to make more progress in the uterine regeneration with the decellularized tissues. For the purpose of rapid migration of the uterine cells into transplanted tissue and vascularization needed for nourishment to the tissue, we removed proteoglycan, a kind of protein in the uterus, from the decellularized tissues with chemical treatment and seeded mesenchymal stem cells into the tissues with the perfusion culture device. We then evaluated them by staining and quantification of DNA and proteoglycan, and confirmed that they has more space into which uterine cells can migrate and distribution of the seeded MSCs.