2D34 Investigation of Permeability of Endothelial Cell Monolayer by Using Microfluidic Device with Controllability of Oxygen Tension

Abstract

Permeability changes of an endothelial cell (EC) monolayer are related to many diseases. This study evaluated permeability of the EC monolayer under normoxia and hypoxia by using fluorescent dextrans of two different molecular weights in a microfluidic device with controllability of oxygen tension. As the results, permeability of the EC monolayer increased by hypoxic exposure, and the increase of the permeability measured by fluorescent dextran of 10 kDa was smaller than that measured by fluorescent dextran of 70 kDa. The results indicated that the size-selectivity of EC monolayer became weaken under hypoxia.