Abstract
The main purpose of this study is the clarification of the effect of substrate elastic modulus on the metabolic behavior of osteoclasts on PCL substrates, and investigation of osteoclastic behavior of HAp/PCL substrates, aiming to extend the possibility to design the substrate elastic modulus. Here, PCL substrates with different elastic modulus were prepared by changing cooling method. The evaluated elastic modulus was 0.295 [GPa], 0.406 [GPa] and 0.657 [GPa] for rapid cooled, slow cooled and furnace cooled PCL substrates, respectively. HAp/PCL substrate with elastic modulus of 0.461 [GPa] was also prepared by rapid cooling. As results, the ratio of activated osteoclasts secreting cathepsin K increased with increasing elastic modulus of PCL substrates. In addition, it was suggested that osteoclasts can produce acid even on HAp/PCL substrates. It is expected that the effect of the composition with HAp on the osteoclastic metabolic behavior will be investigate parametrically, in future.
