ヒト子宮細胞を用いた Scaffold-free tissue の新な作製

Jeonghyun Kim; Tomohiro Matsunaga; Yasushi Hirota; Takehiro Hiraoka; Osamu Yoshino; Shigeru Saito; Yutaka Osuga; Takashi Ushida; Katsuko Furukawa

doi:10.1299/jsmebio.2017.29.2D34

2017.29

Session ID : 2D34

DOI https://doi.org/10.1299/jsmebio.2017.29.2D34

Conference information

Host: The Japan Society of Mechanical Engineers

Name : [in Japanese]

Date : January 19, 2017 - January 20, 2017

Novel method to fabricate scaffold-free tissue using human endometrial stromal cells

Jeonghyun Kim, Tomohiro Matsunaga, Yasushi Hirota, Takehiro Hiraoka, Osamu Yoshino, Shigeru Saito, Yutaka Osuga, Takashi Ushida, Katsuko Furukawa

Author information

Jeonghyun Kim
Department of Mechanical Engineering, University of Tokyo
Tomohiro Matsunaga
Yasushi Hirota
Department of Obstetrics and Gynecology, University of Tokyo
Takehiro Hiraoka
Department of Obstetrics and Gynecology, University of Tokyo
Osamu Yoshino
Department of Obstetric and Gynecology, University of Toyama
Shigeru Saito
Department of Obstetric and Gynecology, University of Toyama
Yutaka Osuga
Department of Obstetrics and Gynecology, University of Tokyo
Takashi Ushida
Department of Mechanical Engineering, University of Tokyo
Department of Bioengineering, University of Tokyo
Katsuko Furukawa
Department of Mechanical Engineering, University of Tokyo
Department of Bioengineering, University of Tokyo

Keywords: endometrial stromal cells, scaffold-free tissue, embryo, early implantation

CONFERENCE PROCEEDINGS RESTRICTED ACCESS

Details

Abstract

Uterus provides an essential place for embryo/fetus to grow inside mother’s body. The study of tissue engineering approaches for uterine regeneration, however, is still limited compared to other organs. In this study, we developed a novel method to fabricate scaffold-free tissue with human endometrial stromal cells (hESCs). We utilized Y27632, ROCK inhibitor, to retain its shape in 3D structure. As well as immunohistochemical assay and Real time-PCR, we also examined its role for early implantation events using rat embryo. It will be expected to imply its potential usage as scaffold in uterine tissue engineering.

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