Abstract
Bone formation is subject in vivo to mechanical stimulation. In order to clarify the effects of mechanical vibration on osteoblastlike cells on the basis of the dynamic properties of the cells, in the sense of vibration engineering, sinusoidal excitation was applied to the cells, and their cell proliferation, morphology and bone matrix generation wete investigated as their frequency response. The cells are cultured under sinusoidal vibration for 24 hours a day, 28 days at frequency of 12.5 or 100 Hz, setting an acceleration amplitude as constant of 0.5 G. The cell density of the vibrating groups has been higher than that of the non-vibrating group after 14-day cultivation by counting the cells and checking their morphology through an optical microscope. The amount of generated calcium salts in the vibrating groups is shown more than that in the non-vibrating group using alizarin red S stain solution on 28-day cultivation. It is found that the bone generation has been promoted by the mechanical vibrating stimulation.
