210 Effects of Vibration Stimulation on Cultured Osteoblasts

Abstract

In order to clarify the effects of mechanical vibration on osteoblast-like cells on the basis of the dynamic properties of the cells, sinusoidal excitation was applied to the cells. The cells were cultured under sinusoidal vibration for 24 hours a day, 28 days at frequency of 12.5 or 100 Hz, setting an acceleration amplitude as constant of 0.5 G The cell density of the vibrating groups was higher than that of the non-vibrating group after 18-day cultivation by counting the cells and checking their morphology through an optical microscope. The amount of generated calcium salts in the vibrating groups was shown more than that in the non-vibrating group using alizarin red S stain solution on 28-day cultivation. It was found that the bone generation was promoted by the mechanical vibrating stimulation.