A Cell Aggregation Method using Acoustic Radiation Pressure and Ekman Transport

(Manipulation of cells using ultrasound and flow)

Abstract

When co-culturing cells outside the body, it is necessary to culture in an environment closer to the living body. In this study, we tried to manufacture a culture vessel and generate three-dimensional tissue of cells by acoustic radiation pressure and Ekman transport. As a result of experiments with particles simulating cells, the particles remained outside the culture surface in the case of only using acoustic radiation pressure. However, by combining Ekman transport and acoustic radiation pressure, particles could be aggregated efficiently. Therefore, three-dimensional tissue of cells can be generated using these techniques.