Abstract
Recently, the bio-ethanol for blend into the gasoline has been focused in order to reduce greenhouse gas emissions. For economical ethanol production using enzymatic hydrolysis and fermentation, the developments of a high concentration saccharification process using a little enzyme and the entire saccharide collecting process from residue after saccharification were demanded. In this study, the saccharificaton using milled sample of Japanese cedar and a solids-liquid separation by the filter press using the residue after saccharificaton were carried out. As a result, the enzyme, one tenth of sample content, maximized the saccharificaiton ratio in case of 2% sample content. The 5% sample content maintained a maximum saccharification ratio. The solid residue collected by the filter-press converted breakdown particles of the sample. And also the solid residue converted breakdown particles in cleaning by pure water.