WS1-4 Visualization of Flow in Microchannels
Details
Flow in rectangular channels on a microchip glass was observed by using a fluorescent microscope with a CCD-video camera. We used Fluorescein as a fluorescent reagent to visualize electrophoretic phenomena and electroosmotic flow. The visualization image of the developing process of the electroosmotic flow showed the mechanisms of electroosmotic flow that ionized samples near the channel wall migrate toward an electrode and drag solvent with them. We also used SYBR Green to visualize DNA migration within a buffer solution. The DNA in the shear flow generated by pressure difference stretched along the flow direction. However, the DNA migrated by electrophoresis were compactly folded structure.
