Abstract
Microbubbles are very small air bubbles with diameters on the order of less than several tens of micrometers. A crucial characteristic of microbubbles is that they are electrically charged on their surface (ζ-potential). In the previous study, we conducted an experiment of the effect of microbubble bathing on organisms by mouse and it was confirmed that microbubbles have an effect on recovering vitality. However, the relationship between restoring vitality and the electrical potential of microbubbles still has not been clarified. The purpose of this study is to investigate the relationship between restoring vitality and the electrical potential of microbubbles. It is assumed that the dynamics behavior of microbubbles can be varies by ζ-potential and the dynamics of microbubbles under ultrasound irradiation was investigated using a high speed microscope.