Improvement of gene transfer using AC electric field induced flow

Abstract

Improvement of gene transfer technology is important to develop a new cell therapy for cancer treatment. The cell surgery technology for gene transfer is developing for several years. We are developing new cell surgery technology for cell therapy. This technology is consisted with three techniques; cell manipulation, gene transfection and cell assessment. These techniques have not been established yet. In this study, the cell surgery device using electrokinetics under a local electric field around a sharp electrode was proposed and confirmed experimentally. Particularly, improvements of gene transfer with AC electro osmotic flow were investigated.