The hydrosphere environment has kept of good conditions naturally by biological purification. However, the natural purification system cannot be maintained anymore in accordance with a dramatic change in life style of modern human society. Therefore, we focused on a water quality depuration system with the aid of micro bubbles. In the present study, we considered the abrupt change of diameter, Ph value and dissolved oxygen value (DO) just before and after generating micro bubbles using a pressurizing dissolution method.