Abstract
Recently, studies of positional relationships of cells are seemed to be very important for cell biology. We proposed a novel concept that self-organized vasculature integrates artificial orifices on a device by VEGF (Vascular Endothelial Growth Factor). Sucking and clamping of Alginate Calcium gel beads containing VEGF, it is able to induce angjogenesis to orifices on embedded channels. Endothelial cells have a characteristic property that they organize vasculature by themselves in collagen gel and grow along the VEGF concentration gradient. HUVEC (Human Umbilical Vein Endothelial Cells) were cultured on device for about 7 days in the collagen gel and artificial vasculature were immobilized on orifices by self-organized.
