An attempt to the measurement of instantaneous pressure field is reported in the wake behind a rectangular cylinder in cross stream. The pressure is obtained by solving the approximated, discrete Poisson equation for pressure, using the velocity field measured by a PIV. The balance in the equation of turbulent kinetic energy is evaluated by the measured instantaneous pressure and velocity field information. The contribution of the pressure diffusion term to the transfer of turbulent kinetic energy is found to be comparable to that by the turbulent diffusion in the wake behind the cylinder.