Abstract
Cells and tissues receive mechanical stimulation such as shear stress and stretching stress. It is known that these stimulation strongly influences the retention of their function. In developing vascular tissues, pulsatile flow culture, mimicking the biological environment, is known to promote the differentiation of BMCs (Bovine Marrow Stem Cells), the production of extracellular matrices, and to increase the mechanical strength of the tissues. However, the effect of the change in frequency on vascular tissue engineering has yet been researched. In this research, we designed culture apparatus that can produce pulsatile flow culture with several frequencies. By culturing with this apparatus, we examined the relation between the frequency changes and the differences in cell number, extracellular matrices, and mechanical stress.
