Examination of liver function by metabolic factors using machine perfusion

Abstract

The donor shortage for organ transplantation is a serious problem. A machine perfusion(MP) is one of the solutions to the donor shortage with recovery and assessment futures. Development of the assessment method of the organ function during MP is the most important issues to expand the donor criteria. In this study, porcine livers, which were harvested after 45 min warm ischemia time(WIT), were evaluated for their functions with metabolic factors (pH, oxygen consumption) during the hypothermic oxygenated machine perfusion(HOPE)). Furthermore, the livers were perfused with autologous blood under body temperature conditions to mimic the reperfusion situation after transplantation. The pH and oxygen consumption during HOPE were compared with conventional biomedical markers( lactate clearance, enzyme, and FMN).