Abstract
In this study, proliferation behavior of osteoblast-like cells, MC3T3-E1, in collagen scaffolds were investigated. For comparison, cells were also cultured on well plates and collagen gels. Variations of cell number and ALP activity were evaluated and it was found that the culture condition using the scaffold exhibited the best increasing behavior. It is also noted that the compressive modulus of the scaffold tended to increase with cell proliferation. a-TCP particle filled collagen scaffolds were also fabricated and it was found that the compressive modulus was effectively improved by addition of a-TCP fillers.
