Systemic chemotherapy is the most popular therapy, especially for breast cancer metastasis. However, a lymphatic administration method has not been developed due to the absence of useful experimental animal models. We have developed a mouse model of the pressure-driven lymph node metastasis via the afferent lymphatic vessel (LV). We developed a system allowing simultaneous imaging of the FITC flow in LNs, and the pressure measurement inside the LNs of MXH-10 mice. Using this system, we also obtained the FITC flow characteristics through the LV, and investigated the administration induced pressure increase, drug dosage and administration speed to deliver drugs to the axillary lymph node from the inguinal lymph node. This administration method requires less doses to deliver drugs relative to the systemic chemotherapy. These results allow more effective and efficient drug delivery system.
