J026014 Quantification of extravasate nano particles due to EPR effect with Window chamber

Abstract

The EPR effect provides a great opportunity for selective targeting of solid tumors. In order to observe the EPR effect, Window chamber (WC) for chronological observation and nano particles that can extravasate from newly-formed tumoral blood vessels have been used. The solid tumor deposition method and the tumor cell injection method are both widely spread of tumor implantation methods into the WC. However, the characteristics of nano particle extravasation dependance on implantation methods have not been reported. Here we show that tumor cell injection method results in a 1.77 higher amount of extravasated particles (per surface area) than solid tumor deposition method by use of fluorescent microscope and fluorescent liposomes with an average diameter of 122 nm. We anticipate our study to help understand the EPR effect in a WC model and gain a better understanding of the EPR effect.