J161045 A cell trap chip having mesh structures fabricated by single-mask inclined photolithography

Abstract

Recently, many types of micro electroporation methods have been proposed for high-efficiency gene transfer technique to living cells. The convensional devices were difficult to operate a large number of cells by a simple operation, and fabicated by the compricated methods with laborious process. In this study , we propose a cell trap chip having mesh structures to operation many cells by a simple operation and a simple fabrication method using single-mask inclined UV lithography. We evaluated the applicability to operate many cells and measure the cell function at single cell level by a cell immobilization test.