In a planar liquid jet with a second-order reaction (A + B →R), the concentrations of reactive species and the streamwise velocity are simultaneously measured by an I-type hot-film probe and an optical fiber probe based on the light absorption spectrometry. The eddy diffusivity of reactive species is obtained from the mean concentration gradient and the turbulent mass flux. The results show that the eddy diffusivity of species A becomes small due to the chemical reaction, and the effects of the chemical reaction on the eddy diffusivity depend on the Damkoler number. These results imply that the gradient diffusion model should be used with considerations of the effects of chemical reactions on eddy diffusivity.