Abstract
This study aims to make clear the effect of directional microstructure of a scaffold on the cell adhesion force. Grooved scaffold was produced by combining lithography and casting of polydimethylsiloxane (PDMS), of which pitch was 1-6 μm and height 0.4-1.1 μm. PC12 cells were cultured and fixed on the scaffold. The cultured cells were peeled-off with a cantilever fixed on a micromanipulator. The adhesion force was quantified by analyzing the deflection of cantilever based on microscopic images captured during peering-off action and the effect of the structural directionality was discussed.
