The Proceedings of Mechanical Engineering Congress, Japan
Online ISSN : 2424-2667
ISSN-L : 2424-2667
2013
Session ID : J026023
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J026023 Estimating trajectories by measuring electrophoretic mobility of MTs
Suguru ANDONaoto ISOZAKITasuku NAKAHARAHirofumi SHINTAKUHidetoshi KOTERARyuji YOKOKAWA
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CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract
Applying kinesin-microtubule (MT) system in vitro as gliding assay provides great possibility of manipulating materials at nanometer scales. Gliding directions of MTs is controlled by electrical field and charges of cargoes. Integrating these techniques, autonomous molecular separation system corresponding to the difference between charges of cargoes is expected to be designed. In order to evaluate the influence of charges of cargoes on gliding trajectories of MTs quantitatively, therefore, we measured electrophoretic mobility of cargo-attached MTs. From this measurement, we estimated gliding trajectories of MTs and evaluated these by calculating curvatures.
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© 2013 The Japan Society of Mechanical Engineers
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