Abstract
A patterning technique of single cells is required for studying cell communication between multiple cells. The objective of this research is to develop a versatile cell manipulation system capable of patterning of single cells in high precision and super parallel format. Here, we investigate cell manipulation by electroosmotic (EO) flow with a micropipette and microprobe-array. Application of DC voltage (5 V) generated an EO flow through the hollow channels of the pipette and probe-array. Cells stained by fluorescent dye, Calcein-AM were captured at the tip of the pipette and probe-array. Cell damage was characterized through the observation of the cells・ In case of micropipette manipulation, a cell swelled and the fluorescence lost 180 s after the cell capture fi°om optical microscope observation. Cells moved toward some of the microprobe-array by EO flow. The cells were tracked and analyzed. Fluorescence of all the cells disappeared 28 s after the voltage application.
