Rolling of neutrophils on vessel wall of post-capillary venules is the earliest event of a cascade of a multistep process of extravasation for the host defense. Since this rolling is considered to be a trigger to initiate the subsequent extravasation process, numerous in vitro studies have been performed on their rolling characteristics using parallel flow chambers mainly focusing on contribution of binding of adhesion molecules on neutrophils and endothelial cells to their behavior. In addition to the binding of the molecules, axially aggregated red blood cells in the vessels are thought to help the neutrophils to be marginated to the vessel wall. Though the pressing force applied to the cells on the vessel wall could enhance possibility of the bond formation of the molecules, the parallel flow camber cannot apply such a force to the cells in the experiment. In this paper, a homemade inclined centrifuge microscope system was introduced. Mechanism of the system to apply a pressing force to the cells was briefly explained. Then, some experimental results of rolling characteristics of HL-60 cell line, which is a well-known model cell of neutrophils, on various types of substrate under the pressing forces were shown.