Recently, cell patterning that enables to make the cell chip has been under study. And in the existing study, two-dimensional cell patterning by transferring cell adhesion solution with inkjet device or photolithography mainly has been studied. On the other hand, to simulate condition in vivo, the three-dimensional cell patterning technique is essential and this enables the construction of more complicated organization in vitro. However the technique to locate nerve cells at the specific position in the three-dimensional gel is not developed. In this study, to develop three-dimensional cell drawing technique and apply it to neural cell chip, we focus on micro injection which can control the three-dimensional position in gel solutions. The three-dimensional cell drawing technology was consisted of micro injector and automatic positioning stage. Polystyrene beads and PC12 cells were located and patterned three-dimensionally in hydrogel. As the results, our drawing system showed possibility of applying the neuronal cell chip.