Abstract
We have been working on the development of scaffold free cell delivery system. Cells construct is made by spheroid-culturing the cells and neatly arrange the spheroids. Therefore, reproducible spheroids formation is required. The spheroids are made by dispensing cell suspension into a special multi-well plate. Then, shape of spheroids depends on the different number of cells and passage number. Therefore, the purpose of this study were to evaluate the spheroids shape made by skilled technician, and to make the spheroids reproducible using the robot developed. We used mesenchymal stem cell of rabbit. Upon reaching the required number of cells, we were dispensed into the special multiwell plate for forming spheroids. We analyzed the quantitative evaluation about spheroids. On the other hand, we incorporated the motion of the skilled technician to the robot developed. Because, in spheroid exfoliation by pipetting or stirring of cells turbid liquid, it is necessary to reconstruct the motions of skilled technicians with specialist knowledge and technique so as not to damage the cells. In result, by skilled technicians techniques be played on the robot developed, we can obtain the spheroids uniformity.
