Abstract
This study proposes a cell subculture method using resonant vibration. The method collects a part of calf chondrocytes from a metal cell culture substrate with resonant vibration of the substrate excited with piezoelectric ceramic plates. We calculated the resonant vibration modes of the cell culture substrate by a finite element method, and conducted eigenvalue and finite element harmonic analyses. Selecting the out-of-plane vibration mode of the substrate, which has a nodal cross, we designed and fabricated the cell subculture device. We then evaluated the remaining and collected cell ratio against the vibration amplitude and the growth response after cell subculture procedure. The proposed method can control the number of remaining cells by changing the amplitude. Moreover, this method statistically improved the proliferation of remaining cells compared with conventional cell subculture process. Therefore, the proposed subculture method could be a promising cell subculture method for adhesive cells.
