The Proceedings of Mechanical Engineering Congress, Japan
Online ISSN : 2424-2667
ISSN-L : 2424-2667
[volume title in Japanese]
Session ID : G2200106
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Cellular-scale Gene Injection to Alive Plants by Micro-Bubble Injector
Sohei ITABASHIShingo MAEDAYoko YAMANISHI
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Abstract

We have succeeded in local and in-situ injection of reagent into a single plant cell by using the cavitation phenomena of micro-bubbles. Micro-bubbles were generated electrically by a novel device called “micro-bubble injector”. This device provides minimally invasive processing and high accessibility compared to conventional injection techniques such as electroporation, sonoporation and particle-gun methods. One of the strong point of this device is injection to the material which has wide dynamic range of hardness. Thus, it enables to applied to plant cells which have been difficult to be injected due to hard cell wall for a long time. This technology contributes to the biological and medical researches developments.

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© 2016 The Japan Society of Mechanical Engineers
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