Host: The Japan Society of Mechanical Engineers
Name : Mechanical Engineering Congress,Japan
Date : September 11, 2016 - September 14, 2016
We have been investigating how to use protists as living micromachines by controlling their behaviors using their taxes. Previously we succeeded to transport an object, to assemble simple mechanical parts and to drive micro seesaw by the force of Euglena group gathered around the position controlled blue laser beam. The generation mechanism of Euglena group, however, is still unknown since the group formation was only seen in the high cell density culture solution and in the low density culture, Euglena did not gather or it took very long time to form the group. It means that the behaviors of the individual Euglena in the high cell density culture did not equal to that in low cell density condition. In this paper, we develop an analyzing program of every Euglena's moving traces even in the high cell density condition. The results showed that it needs laser irradiation experience for Euglena to gather around the laser beam. It was clearly confirmed by the experiments of the Euglena's reaction to the moving laser. In this case, only the Euglena behind the laser move to the laser beam. This fact gives an explanation why Euglena does not gather or needs very long time to gather in the low cell density condition. It is that the laser irradiation probability to each cell is very low in the low cell density culture condition.
