Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : September 09, 2018 - September 12, 2018
For the purpose of application to biosensors, we newly characterized the electrical properties of the λDNA molecules. After the λDNA molecules were introduced using a microchannel measuring 50 μm in depth and 500 μm in width, they were electrostatically stretched and immobilized between two triangular-shaped aluminum electrodes with a gap of 14 μm by applying an AC voltage of 1 MHz and 20 Vp-p. In order to reveal the detailed electrical properties such as parasitic resistance, and parasitic capacitance which are attributed to the conductivity of λDNA molecules, we evaluated the impedance frequency characteristics of the λDNA molecules using EIS (Electrochemical impedance spectroscopy) measurements. From the complex impedance of the λDNA molecules, an equivalent circuit was obtained as a series connection of two parallel circuits consisting of two resistances and two parasitic capacitances. The DNA molecules which were immobilized and evaluated in this study can be applied to electrical detection of deoxyribonuclease (DNase), enzyme for nonspecific DNA cleavage, which is a candidate biomarker for acute myocardial infarction.
