The Proceedings of Mechanical Engineering Congress, Japan
Online ISSN : 2424-2667
ISSN-L : 2424-2667
[volume title in Japanese]
Session ID : J1510303
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Influences of Diameter and Position of the Magnet on Label-free Diamagnetic Cell Patterning
*Yutaka HISHIDAYoshitake AKIYAMA
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Abstract

We aim to develop the label-free diamagnetic cell patterning system by magnetic scanning which based on the magneto-Archimedes effect. By adding the paramagnetic compound to the fluorescent beads suspension, fluorescent beads were pushed away from the trajectory of a magnet. Based on this method, the system is able to pattern the micro particles including cells by magnetic scanning. In this study, we patterned the fluorescent beads on the substrate and examined to control the size of the patterned fluorescent beads area where the fluorescent beads were removed. The patterned area was determined by image analysis. As a result, the fluorescent beads above the magnet were pushed away by diamagnetic force and patterned in the circular shape on the bottom of the chamber. As the magnet became wider or approached the bottom of the chamber, the patterned area enlarged. In conclusion, this study has demonstrated that diameter and position of the magnet affected the size of the patterned area.

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© 2018 The Japan Society of Mechanical Engineers
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