Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : September 08, 2019 - September 11, 2019
Cell rolling on the vascular endothelium is an initial phenomenon of allergy, arteriosclerosis, or cancer metastasis and detailed understanding of this phenomenon would help the development of innovative treatments for these diseases. In this study, we observed the rolling behaviors of leukocytes on E-selectin-coated microchannel walls with various flow rate and different E-selectin density. As a result, we found that the rolling motion can be sorted into two modes from a perspective of the instantaneous cell migration. The first mode (Mode 1) is characterized by rather intensive rolling with low probability, whereas the second one (Mode 2) is characterized by mild rolling with high probability. We also found that thresholds of the mode are determined by both the wall shear stress exerted on the cells and the density of E- selectin on the wall. Furthermore, it was implied that the force to untether the adhesion molecules bonds by the flow is larger in Mode 2 than in Mode 1.
