Development of cell counting module using soft lithography technology

Abstract

One way to ensure the reproducibility of cell experiments is to automate the workflow, which can be achieved using microfluidic device technology. However, microfluidic device technology is only used in a few studies because it requires a lot of time and money, in addition to the know-how needed to develop the technology. In this study, modular microfluidic devices that are inexpensive and freely combinable were developed by integrating micromilling and soft lithography technologies, and the performance of a prototype cell counting device was evaluated. The combination of such single-function devices (modules) and the use of machine learning is expected to improve the efficiency of microfluidic device development.