Abstract

Analysis using multiple reagents, such as enzyme-linked immunosorbent assay (ELISA) in microfluidic paper-based analytical devices (μPADs), requires fluid control in paper channel. In this study, a valve mechanism using thermo-responsive polymer was developed as a fluid control technology applicable to μPADs. To demonstrate fluid control of multiple solutions with μPADs using thermo-responsive valve, fluid control test was conducted with two different coloring liquid. However, fluid control couldn’t be achieved because the valve didn’t operate properly during the test. To investigate the condition under which the valve can properly perform its open and close function, we evaluated the relationship between the heating time, temperature, and valve performance. As a result, we confirmed that the valve could retain its closing function afterwards if heated at 45℃ or less for 15 minutes or less.