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This paper concerned with the functional design of fish scales. The rough surfaces of fish scales were measured with a three-dimensional, optical shape measuring system. The measuring system consisted of the light microscope system, laser displacement detector system, automatic scanning XY stage system and automatic focusing system. The surface roughness of fish scales can be measured with μm order accuracy. The scales of a shark, Mustelus manazo Bleeker, were also observed microscopically waking use of the scanning electron microscope. The microscopic structure and morphological characteristics of fish scales were revealed for some fishes.