Pages 267-268
We have constructed a capillary electrophoresis (CES) system using living cells as the sensor tip of CES to monitor the change of cellar environment. In the present system, yeast cell stained with fluorescence dye was applied as the sensor cell. Fluorescence intensity of the probe cell changes according to the change of cellar environment. To confirm the performance of the proposed CES system, the pollutant (Hg^<2+>) and the biological substance (acetylcholine) were applied. Fluorescence intensity of Rhodamine123 (membrane potential sensitive dye for mitochondria), decreased gradually due to effect of the pollutant, while that of Fluo-3 (calcium sensitive dye) increased.
