Abstract
The accurate prediction of intracellular ice formation in biological cell is greatly important for determining life-death of living cell. In this paper, by combining the artificial biomembrane and two chamber as intra- and extra-cell, simplified biological cell was simulated. As biomembrane, phospholipid bilayer formed by folding technique was used. Summarizing experimental results, the barrier effects of cell membrane foe seeding by extracellular ice was clarified in conjunction with the following parameters; cryoprotectant in extra- and intra-cell, space between phospholipid molecules, and osmotic stress.
