This research studies on the acceleration mixture and chemical reaction of two reacting fluids by using special structure in a micro-fluidic device. By using this structure, the flow of two reacting fluids becomes four layers and the reaction speed becomes faster than usual two-layer flow. The typical flow channel width of a micro-fluidic device is 40 to 80 μm. This size is not easy to fabricate or to visualize the flow. First, we experiment with the flow channel 10 times as large as the actual device to overcome these difficulties. Paints were used as the visualization method of the flow. The experimental result showed that in order to increase mixture and chemical reaction at high velocity, the number of layers increases further and mixing speed must be faster. For that purpose, the structure of flow channel is a problem. In order to solve the problem, the analysis and the simulation in CFD performed.